It is very useful dear Edelmann. I highly appreciate your assistance.

Thanks a lot

Dear Yasser Fakri Mustafa, please find attached a patent on this topic which might give you some idea. I hope it is useful.

It is very useful dear Edelmann. I highly appreciate your assistance.

Thanks a lot

Dear Yasser Fakri Mustafa, you're very welcome. I'm glad to hear that the information was helpful. 👍

I am the gladdest. Thank my professor.

Methanol or Ethanol

Thanks. You mean polar protic solvents?

polar protic solvents

Acetat de sodium ansi que le n-butanol

Thank you Dear Ghada. You mean sodium acetate and n-butanol or sodium acetate aqueous solution or n-butanol?

Dear Yasser Fakri Mustafa, attached please find another potentially useful article in which the extraction properties of different solvents are compared.

Actually, this report is highly valuable. Thank you dear Edelmann, you are always direct me toward the right way.

Flavonoids are polar compounds having at least 3 or more Hydroxyl groups at position 3,5,7. The hydroxyls can be prenylated leading to reduced polarity and there can be attachments of sugar(s) leading to increasing polarity. So to extract flavonoids in general, you need moderately polar solvents (chloroform, ethyl acetate etc) which have affinity for the less polar flavonoids or highly polar solvents (acetone, butanol or sometimes ethyl acetate) which have affinity for the highly polar flavonoids. It can be successive extraction or cold maceration follow by partitioning with hexane, chloroform, ethyl acetate and butanol in order of polarity. Since the sample is leave, you can defatt with hexane first before the normal flavonoid extraction. Good luck.

Dear Adebayo

Thank you very much for these helpful information.

Regards

According to my knowledge, mixed solvents like aqueous ethanol or methanol are the most widely used for the extraction of flavonoids due to higher yields achieved in the recovery of flavonoids. Furthermore, it is advisable to use the echo-friendly modern methods for extraction of flavonoids like microwave-assisted or ultrasonic assisted extraction method which offer many advantages like reduction of extraction times and solvent volume with higher extraction yields of the flavonoid compounds than conventional methods.

Dear Mahmood Khudhayer Oglah

Thank you very much for your answer.

Regards

Dear all, without specifying the type of flavonoid and which plant is to be extracted, only general suggestions are supplier. These links are for similaire threads on RG. My Regards

https://www.researchgate.net/post/How_to_isolate_flavonoids_from_plant_leaves#:~:text=The%20less%20polar%20solvents%20(ether,et%20al.%2C%201970).

https://www.researchgate.net/post/how_to_to_extract_and_purify_the_flavonoids_from_plant_sample

Thank you very much dear Abdelkader BOUAZIZ

It is a valuable note.

Regards

Hi;

You can use distilled water and ethanol 70%.

With my best regards

Thanks dear Kou

Using one solvent or mixture of solvents to extract flavonoids is related to your plant material in addition to other factors that affect extraction. In general, the use of medium to high polarity solvent is convenient to extract flavonoids.

Dear Noureddine Boulenouar

Thank you very much. Can you specify which solvent exactly recommend?

Regards

Dear Yasser Fakri Mustafa,

The polarity chart attached below may be useful to choose the convenient solvent or mixture. The polarity indexes in second column. Also, miscibility character is important when preparing mixtures.

Regards

Dear Noureddine Boulenouar

Thank you very much

All the best

Kindly check this link

Evaluation of the Use of Different Solvents for Phytochemical Constituents ...

https://www.hindawi.com/journals/jfq/2019/8178294/

Methanol is one of the most effective and efficient solvents for the extraction of flavonoids from plant leaves

Check this useful link

Comparison of Different Solvents for Phytochemical Extraction Potential from ...

Methodology: To achieve this, different extracts from the plant leaves were made ... Using methanol as an extraction solvent results in the maximum flavonoid extraction with ...

https://scialert.net/fulltext/amp.php?doi=ijbc.2017.17.22

Dear Chinaza Godswill Awuchi

Thank you very much

Regards

Please review the following articles contain excellent information:

https://www.scielo.br/pdf/cta/v37n3/0101-2061-cta-1678-457X23516.pdf

file:///C:/Users/FT8/Downloads/processes-08-00434.pdf

Dear Tawfiq M. Al Antary

I find it. I will review this paper thoroughly. Thank you very much

All the best

Dear Omar

Thank you very much. By reviewing these files, I find them very useful.

Regards

Dear Tawfiq M. Al Antary

Regards

This link is also relevant

Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves by V Sharma

Isolated compound was dissolved in respective solvent CDCl3 and about 600 μl was poured in NMR tube and observed on the applied ...

https://www.sciencedirect.com/science/article/pii/S1878535214001816

Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant ... by TV Ngo

The mixture of 50% (v/v) water with acetone and ethanol had the highest extraction yields of flavonoids (100 and 89 mg CE/g DW, resp.)

https://www.hindawi.com/journals/jfq/2017/9305047/

Dear Chinaza Godswill Awuchi

Thank you for your valuable contribution.

Regards

The best solvent or solvent mixture for extracting flavonoids from a plant leaves

is ethanol which is polar and cheaper solvent.

Dear Madhukar Baburao Deshmukh

Thank you very much.

Regards

I agree with Madhukar

The best solvent or solvent mixture for extracting flavonoids from a plant leaves

is ethanol which is polar and cheaper solvent.

Dear Abdelkader Dehbi

Thank you for your answer.

Regards

I think; benzene, chloroform, and ethanol.

Dear All, I don't know if benzene is a good solvent for this purpose. In any case I would strongly suggest to avoid working with benzene because of its high toxicity. If at all possible, you should replace it by toluene or other suitable organic solvents!

Dear Frank T. Edelmann

Thank you very much for this note.

Regards

Dear all, I agree with Prof. Frank T. Edelmann, benzene belongs to a family of solvents abbreviated as BTEX which stands for: benzene, toluene, ethylbenzene, and xylene, they are highly toxic and strongly presumed carcenogenic. They are disregarded in many applications such as paints and adhesives, for the aforementioned high potential danger they present. My Regards

Dear Abdelkader BOUAZIZ

Thank you very much for your kind contribution.

Regards

Dear Aws Maseer Nejres

Thank you very much for your kind contribution.

Regards

Dear G Srinivasarao

Thank you very much for your kind contribution.

Regards

Schiff bases using 2-amino-4-methyl benzothiazole can be synthesized by refluxing equimolar proportion of 2-amino-4-methyl benzothiazole and aryl aldehyde in methanol in the round bottomed flask till solid Schiff,s base separates out in the flask as a solid.Add 2,3-dropd of acetic acid during refluction. ref-Text book of Practical Organic Chemistry by A.I.Vogel.

Dear Madhukar Baburao Deshmukh, I think your answer is not at the wright place. It is for another question, and you have already participared. My Regards

https://www.researchgate.net/post/Is_it_possible_for_obtaining_the_Schiff_bases_using_2-amino-4-methyl_benzothiazole#view=5ef099ed29fa28752e6d1c30

Ethanol

The best is to use ethanol, but in the beginning the lipid part is removed using hexane solvent and then extract flavonoids using ethanol

Dear Madhukar Baburao Deshmukh

Thank you very much.

Regards

Dear Abdelkader BOUAZIZ

Thank you for your note.

Regards

Dear Muhammad Akram

Thank you for your contribution

Regards

Dear Luay_Al-Helaly

Thank you very much for your kind contribution.

Regards

I would suggest that methanol is the best and most effective solvent for extraction, resulting in the highest extraction yield and the highest content of phenol, flavonoid.

Best.

Methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy. Dieu-Hien Truong et al, 2019, Journal of Food Quality, Volume 2019, Article ID 8178294.

Dear Mehdi Shakibaei

Thank you very much for your kind contribution.

Regards

Phytochemicals: Extraction, Isolation, and Identification ... - MDPI

www.mdpi.com › pdf

PDFSep 22, 2017 - such as flavonoids, tannins, and lignins, found in plants, all act as antioxidants [3]. ... Solvents used for the extraction of biomolecules from plants are chosen based on ... a good technique in multiple fields, especially in the medicinal plant area. ... classes of compounds in both pure and biological mixtures.by A Altemimi - ‎2017

Dear Abhijit Mitra

Thank you very much for your kind contribution.

Regards

As far I can understand, the performance of solvent or solvent mixture primarily depends on the types of plant leaves used for extraction of flavonoids. In general, methanol could be a good choice.

Dear Sakil Mahmud

Thank you very much for your kind contribution.

Regards

According to the literature, the most effective solvent for separating flavonoids from plant materials is 70% aqueous ethanol.

Dear Sabir Nishanbaev

Thank you very much for your kind opinion and contribution.

Regards

As far as extraction is concerned, a multi-step approach is required.

Organic solvents are definitely going to dominate the whole process!!

Chloroform is good for Flavonoids

Ethanol and Acetone are good for Flavonols

Methanol is good for Flavones

Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w).

The alcohol of high concentration (90–95%) is applied to extract free flavonoids, and the alcohol at the concentration of about 60% is applied to extract flavonoid glycosides.

Chapter Isolation and Structure Identification of Flavonoids

Dear Omkar S. Kushwaha

Thank you very much for your kind opinion and contribution.

Regards

Dear Hussein J. Hussein

Thank you very much for your valuable opinion and contribution.

Regards

Dear Aijaz Panhwar

Thank you very much for your valuable opinion and contribution.

Regards

Dear Ali Esmail Al-Snafi

Thank you very much for your valuable opinion and contribution.

Regards

Dear I. Abu-Reidah

Thank you very much for your valuable opinion and contribution.

Regards

Dear sir,

It is better to use ethanol and water (9:1) for extraction and non polar, non polar- polar solvents in increasing order for separation of flavonoids using chromotography.

Dear Dr. Jeelan Basha

Thank you very much for your valuable opinion and contribution.

Regards

in general flavonoids are polar compounds, so they can be easily extracted using polar solvents such as ethanol, methanol and acetone. the addition of water in the extraction system is beneficial for the extraction of flavonoids with higher molecular weight. conversely to acetone, ethanol and methanol allow the extraction of an important quantity of sugar and protein... that could interfere with the dosage of flavonoid. otherwise, there is no universal extraction method for flavonoid, so it is better to optimize your extraction method using different solvent extraction,

Dear Youness Ennahli

Thank you very much for your valuable opinion and contribution.

Regards

Ethanol 80%

Ethanol, methanol even hot water. It depends on the kind of flavonoid that you're looking for.

see the attachment below