I've tried a few different programmes (Image J, Optimus, Photoshop) but I would be interested in other researchers opinions. Has anyone found a program that does a good job of automating increment width measurement?
Try KS Runs (Carl Zeiss Vision). It will allow you to write a simple macro that will automatically recognise and measure otoliths from suitable image files.
Hi. ImagePro Plus seems to be the standard nowadays. However, the best software in my opinion is sold in Japan (but has an English version): RATOC (http://www.ratoc.co.jp/ENG/jiseki.html). Quite expensive though.
Photoshop (extended version) is useful, and can integrate well with other Adobe products. Otherwise, Image Pro Plus is being used routinely here. Good luck.
Dear colleague, did you know the Age & Shape from (Morales-Nin group) from Infaimon? Cheers
Dear colleague here give you link for cheking some software to measure productivity http://www.uq.edu.au/economics/cepa/
I second Age&Shape by Infaimon. It can automatically detect increments according to rising, falling or midpoint greyscale intensities. The positions of the automatically detected markers are completely manipulable. Markers can also be positioned manually and you can measure distance between them. It's a little expensive and still has a few glitches but you get two features for the price of one in that it has morphometric functions too.
well I suggest you to use Stochastic Frontier approach.
Photoshop (extended version) particularly CS4 and CS5 does well for me. Non-extended versions do not have the calibration and measurement options. I worked a bit with imageJ but i only use it to confirm my finding from the use of photoshop.
Apparently this ImageJ tree-ring macro works for otolith rings as well. It didn't do a good job with daily growth, but could be useful for annual growth.
alshttp://simon.bio.uva.nl/objectj/examples/TreeRings/TreeRings-9.htm
We use TNPC, which has been developed by Noesis and IFREMER specifically for working with calcified structures. It's very effective for increment width measurement and growth pattern analysis. See http://archimer.ifremer.fr/doc/00032/14288/.
You might want to take a look at Image-Pro Premier software and their add-on otolith app developed by Alaska Fisheries. Image-Pro is expensive, but very nice.
Hi. There was a software called OTO some years ago, working on live (video) images. This software is not working any more and we are now trying to develop a new live software with some engineer collaboration. Althout it is not working yet probably we will have a prototipe nest year. By now were are using TNPC on microstructure photographs. Please, if you try a better way to read daily growth let us know.
We use Image-Pro Plus V7. Its straightforward, but, as previously mentioned, expensive. In my experience with Image-pro Plus stitching of multiple images together (called tiling in image-pro) is cumbersome and at times frustrating. 'Live tiling' might be of interest if you have the need to combine multiple otolith images. (http://www.mediacy.com/index.aspx?page=Otolith)
Image-pro plus does a good job, but I found it a bit cumbersome. ImageJ allows you to easily record macros, so its very simple to make your own algorithms up. This function also has the advantage that its easy to export the data by making it produce a .xls or .txt file of the data once the measurements are analysed (if you set it to export into a temporary directory you can then automate upload into a database).
Try KS Runs (Carl Zeiss Vision). It will allow you to write a simple macro that will automatically recognise and measure otoliths from suitable image files.
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