Inserted gene gives band but not for plasmid in colony PCR for cloning?

Hello, After cloning, we chose the white colony from IPTG and Xgal agar, then cultured in LB broth and do PCR from that broth. We found the bands for our inserted gene, but not for plasmid. We use...

05 June 2019 4,501 9 View

Is it possible to use SSR to investigate the bacterial diversity in soils?

07 August 2017 3,257 5 View

Could biofertilizer replace the use of chemical fertilizer in developing countries?

In our country, most farmers are using chemical fertilizer to improve crop yields. Researchers from Biotechnology Departments are trying to produce effective fertilizer to reduce the use of...

01 January 1970 1,649 64 View

Can someone advise on how to deactivate genes in phosphate solublising mechanisms of Bacillus megaterium?

I would like to know genes that control the Phosphate solubilizing mechanism in Bacillus megaterium in detail. I would like to do site directed mutagenesis to deactivated gene in phosphate...

01 January 1970 9,651 0 View

I would like to get some literature about the differences of carbon fixation pathways in different soil types. Could someone help me?

Hi there! I would like to get some literature about the differences of carbon fixation pathways in different soil types. Could someone help me? I need some papers to cite. Just links to download...

01 January 1970 2,219 16 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View