Histopathological changes in different organs are generally assessed to find the health condition of fish.I wish to know which magnification I should see to find if there are any changes in normal histo-architectural changes in different organ
Enhanced histopathology (EH) of the immune system is a tool that the pathologist can use to assist in the detection of lymphoid organ lesions when evaluating a suspected immunomodulatory test article within a subchronic study or as a component of a more comprehensive, tiered approach to immunotoxicity testing. There are three primary points to consider when performing EH: (1) each lymphoid organ has separate compartments that support specific immune functions; (2) these compartments should be evaluated individually; and (3) semiquantitative descriptive rather than interpretive terminology should be used to characterize any changes.
Dear Sir,
I am unable to understand your answers. Can you please write a elaborate answer to my query in a comprehensive manner.
It depends on many factors like organ, tissue, staining method and type of used microscope.
Regards
SM Najim
Dear Sir,
I wish to do histopathological study of fish liver and intestine and i have used H& E stain. Can you please now suggest answers to my questions.
If it is routine histology your choice(I mean Liver and intestine) is good.I would add -kidney and pancreas. And you have to do different stains-for example Sudan black stain for fat cell(hepatic lipidosis) or Alcian blue or other stains.And of course i need control fish for comparison to experimental fish.
Try Ole B Dale at the Norwegian Veterinary Institute. He is a skilled histopathologist, now working on a project on intestinal health.
Thank you Camilla Fritsvold for adding the answer to my query. Can you please help me to find his contact, email address.
Histopathology is primarily about pattern recognition. You are looking for changes in structure and colour that indicate a deviation from the norm. So I generally scan the slde first with a x20 objective so that I can quickly locate any significant lesions, then I scan the areas of interest with a x40 objective (the best that I can afford) and I take time - 5 minutes average per slide. I use the x100 oil only for resolving fine detail that the x40 will not show clearly.
Associated with this is the need to know what is "normal" because tissues change with the physiology of the animal, build up a reference set of "normal". I also I spent some time with the histologist learning what artifacts look like (She made me sets of slides with deliberate errors - understaining, overheating etc. Great fun!).
Finally, it is really important to participate in a regular "show and tell" session with fellow pathologists and shre your findings. That way you learn off each other.
Remember that the pictures you see in books and papers are not the average - they are extreme examples used to prove a point. Reality is never so obvious.
The European Association of fish pathology histology workshop in September 2017 in Belfast will include a lecture by Dr Jeff Wolf, an expert on artefacts in fish histology. Hopefully his presentation will be published.
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