T-test and Mann-Whitney U test on groups with different sample sizes and variances?

Hello I have 2 groups with different sample sizes of (experts: 5 to 8) and (Users: 28 to 35) answering 21 questions on a 7 points Likert scale about the importance of some dimensions for...

31 January 2021 3,885 7 View

What are the types of optimization problems that can't be tackled by meta-heuristic and heuristic algorithms?

Any decision-making problem when precisely formulated within the framework of mathematics is posed as an optimization problem. There are so many ways, in fact, I think infinitely many ways one can...

06 September 2020 9,415 14 View

Is it possible to reduce tensile strength and increase compressive strength by adding any admixture to concrete?

Hello, I want to know is it possible that the compressive strength of concrete increase by reducing tensile strength? if yes, how we can explanation and justification this issue?

30 April 2020 4,489 7 View

What are the plans for exams and assessments of universities during corona virus pandemy?

What alternative ways universities use for academic assesment of students?

29 April 2020 3,091 1 View

Is there a specific way to discretize a continuous model to any arbitrary multi DOF system and extract the mode shape matrix in abaqus?

27 April 2020 239 3 View

What is best sampling method for cross sectional studies?

I want to research on liver disease patients behaviours and habits in shiraz city. How can I calculate sample size accurately? Thanks

23 March 2020 9,974 5 View

Anyone in kolkata working of LNCaP prostate cells?

I am working with prostate cancer cells, i want to know who is presently working with PCa cell lines in viro and in vivo in kolkata.any leeds will of great help.

07 March 2020 7,831 1 View

How can I find the IDT point of the following TGA curve which is related to a clay soil?

The attached file shows the TGA curve of a clay soil. Can anyone help me how to recognize the initial decomposition temperature (IDT) from this curve?

06 February 2020 6,944 3 View

How to isolate dorsal root ganglia in rat at lumber region?

For pathological investigation of sensory and motor nerves in lumber region of rat how to isolate and preserve

04 November 2019 8,974 1 View

What's the procedure for validation test of a new design of product (Say Heat Exchanger)?

after 3D design of all components of the Heat Exchanger geometry we do assembly and finalization of the geometry, in CFD analysis we change the boundary conditions and it refers us to the...

27 October 2019 9,095 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View