What is the best protocol to grow and store amoebae isolated from water sources?

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Rita Smirnov

KALININA L.V., PAGE F.C. 1992: Culture and preservation

of naked amoebae. Acta Protozool. 31: 115–126.This article may help you.They recommended to use Non-nutrient amoeba saline agar(NN) and MY75S agar.Also Dr.Iva Dikova has many useful articles about fish amoeba

Joseph Caruso

You can culture them on Enterobacter spp. on agar plates.

Aline Perrin

Or on non-nutrient agar (NNA) plates with living or heat-killed E.coli. The incubation temperature is generally function of amoebic strains.