the bacterial ligninolytic potential is still largely unexplored and ... After seven successive transfers to fresh lignin medium, individual colonies were ... In addition, various standard biochemical tests and cellular fatty acids. Bacteria either utilize the pesticides as sole carbon sources or, in some  cases as sole nitrogen sources, or alternatively co-metabolize them. Genetic studies of pesticide degrading bacteria have centered on cloning and characterization of specific enzymes involved in pesticide degradation. The involvement of plasmid-encoded catabolic sequences in pesticide

degradation has been widely documented . The tubes are  incubated at 37oC for 15 minutes. The blank contained substrate and crude enzyme which are inactivated by boiling. The optical densities of the tubes recorded for the amount of enzyme activity at 530 nm.In order to optimize the culture condition the pH of the medium is  adjusted to desired value by addition of either 0.1M HCl or 2, 5 M NaOH to reach the proposed value. Laccase activity is  estimated at pH 3- 11. To obtain pH 3,Glycine - HCl buffer (0.2M), for pH 4 and 5 acetate buffer at 0.2M concentration, for pH 6 and 7 phosphate buffer (0.2M), for pH 8 and 9 Glycine – NaOH buffer (0.2M) and carbonate- bicarbonate buffer (0.2M) for pH 10, 11 are used

Hi,

In this article you will find 2 assays to detect lignin degradation by bacterial strains:

https://www.researchgate.net/publication/44692217_Development_of_novel_assays_for_lignin_degradation_comparative_analysis_of_bacterial_and_fungal_lignin_degraders

Cheers,

vincent

Article Development of novel assays for lignin degradation: Comparat...

LB agar supplemented with 20 mg MB/100 ml used to study decolorization. it is indication of lignolysis