What is the best protocol available for the molecular detection of Plasmodium falciparum gametocytes?

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Andrea Ruecker

Hi Rashad,

as mainly the more mature stages can be found circulating, a common way of detection is using the Pfs25 QT-NASBA.

Andrea

Rashad Abdul-Ghani

Hi Andrea,

Many thanks for your your kindness. I am a little bit confused about choosing a protocol for detection of Plasmodium falciparum gametocytes. As a quantitative approach, QT-NASBA is the preferable choice. Is Pfs25 better than Pfs16? I would appreciate your help if you can send me a detailed protocol for QT-NASBA for P. falciparum gametocytes.

With my best regards,

Rashad

Dear Andrea,

What about using reverse transcriptase-PCR? Practically, what are the advantages and disadvantages of RT-PCR and QT-NASBA for the detection of P. falciparum gametocytes?

Best regards,

Habibollah Turki

Hello My dear Rashed

Nested-PCR as a sensitive and specific method for malaria diagnosis which can detect low parasite densities.

Best regards.

My dear Rashed Abdul- Ghani

Thank you for your contribution, dear Habibollah. For the detection of gametocytes, we have to target RNA rather than DNA. Therefore, we have to adopt strategies other than nested PCR.