What is the best Proteomics-Compatible Lysis Buffer for isolated nuclei?

Mervi T Hyvönen @Mervi_Hyvoenen

01 January 2014 1 10K Report

It needs to solubilize also the nuclear membranes. Isolated proteins are to be analyzed with 10-plex mass spectrometry.

Mervi T Hyvönen

Thanks! I'll try that.

Badges
Science topic

More Mervi T Hyvönen's questions See All

How to differentiate 51 and 55 kDa protein isoforms?

I have observed expression of two splice variants, which should code protein isoforms of 51 and 55 kDa. I have antibody for the protein, but is there any conditions for SDS-PAGE to differentiate...

05 June 2019 6,124 11 View

Enzyme pH optimum in vitro vs. subcellular localization

Why does a cytosolic enzyme (pH~7.4) have an pH optimum of 9.0 in vitro (recombinant protein)?

05 June 2017 3,994 11 View

Hormone content of FBS? Insulin depletion from FBS?

Does anyone know where to find the list of average concentrations of hormones in FBS? I know that insulin is about 40 pM in 5% FBS, but what about the other hormones? And does anyone have a...

03 April 2013 6,578 8 View

Protocol for Slab-IEF?

Does anyone have a protocol for slab-IEF for minigels (1.5 mm thick, 7cm)? After IEF I would like to transfer the proteins to membrane and do immunoblotting (without second dimension run). The pI...

01 February 2013 2,301 9 View

How can I prevent isolated human adipocytes clumping to each other?

What should I add to the buffer after collagenase digestion? I'm now using 1% BSA/200 nM adenosine/PBS to wash the cells. After staining with DAPI and membrane marker I mix 1:10 with glycerol and...

01 February 2013 8,613 4 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

My question is about a barley seed ?

I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.

26 June 2024 3,451 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How to handle missing data for proteomics data?

I have a proteomics dataset with missing values. I tried some strategies, but the point is that there are sets completely with missing values. The last strategy was to apply MissForest in python...

29 May 2024 5,563 4 View

Are there any international companies that provide LC-MS/MS proteomics services to whom we can outsource our samples??

We want to find interacting protein partners for our protein of interest.

28 April 2024 921 1 View

Can MaxQuant be set up to detect sodium ionisation of peptides?

Due to factors outside of my control, peptides in my ESI-MS data have been ionised normally by protons ([M+2H], [M+3H]...) but also by sodium ([M+2Na], [M+3Na]...). Is there a way to configure...

16 April 2024 10,028 2 View

Can I use hypotetical proteins as a novel peptide?

Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...

04 April 2024 5,098 2 View

How many ug/particles of EVs is needed for doing proteomics?

How many ug/particles of EVs is needed for doing proteomics? We are doing many steps for purify EVs of human plasma and at the end of the process we are getting just 1/2ug/ml concentration of...

25 March 2024 730 1 View

In serum proteomics, we add 15% TFA to stop trypsin activity. How does TFA stop trypsin activity?

serum proteomics

17 February 2024 1,086 3 View

How to proceed when technical repeats (different injections in Mass Spec) are scattered and cluster with other samples in PCA?

Could this be due to an error in Mass Spec calibration or data analysis? I have 2 technical repeats that are fine, but the 3rd repeat is far away in the PCA plot and clusters with replicates of a...

05 February 2024 5,464 3 View