Mervi T Hyvönen

6 Questions 16 Answers 0 Followers

Questions related from Mervi T Hyvönen

How to differentiate 51 and 55 kDa protein isoforms?

I have observed expression of two splice variants, which should code protein isoforms of 51 and 55 kDa. I have antibody for the protein, but is there any conditions for SDS-PAGE to differentiate...

06 June 2019 6,107 11 View

Enzyme pH optimum in vitro vs. subcellular localization

Why does a cytosolic enzyme (pH~7.4) have an pH optimum of 9.0 in vitro (recombinant protein)?

06 June 2017 3,978 11 View

What is the best Proteomics-Compatible Lysis Buffer for isolated nuclei?

It needs to solubilize also the nuclear membranes. Isolated proteins are to be analyzed with 10-plex mass spectrometry.

01 January 2014 9,644 1 View

Hormone content of FBS? Insulin depletion from FBS?

Does anyone know where to find the list of average concentrations of hormones in FBS? I know that insulin is about 40 pM in 5% FBS, but what about the other hormones? And does anyone have a...

04 April 2013 6,564 8 View

Protocol for Slab-IEF?

Does anyone have a protocol for slab-IEF for minigels (1.5 mm thick, 7cm)? After IEF I would like to transfer the proteins to membrane and do immunoblotting (without second dimension run). The pI...

02 February 2013 2,284 9 View

How can I prevent isolated human adipocytes clumping to each other?

What should I add to the buffer after collagenase digestion? I'm now using 1% BSA/200 nM adenosine/PBS to wash the cells. After staining with DAPI and membrane marker I mix 1:10 with glycerol and...

02 February 2013 8,598 4 View