Hello, you can do by retinoic acid or with serum deprivation.

Hello, you can do by retinoic acid or with serum deprivation.

Karen A. Darbinyan

Hi dear karen

thanks for your answer

I know that i should use retinoic acid or serum deprivation for induction of differentiation but i don't know which marker is the best for evaluation of the differentiation process at different time points

Hello

Yes, you can do this with either 20 uM Retinoic acid .

Take a look on this question and PDF

https://www.researchgate.net/post/What_are_the_details_of_N2A_cell_differentiation_methods

Article Differentiation of N2a cells into dopamine neurons.

Good Luck

did you get any candidates for evaluating differentation?

im also looking for any markers can be used with western blot and qPCR analysis.

now i make a list as MAP-2, tubulin 3 and NeuN for western, and Chat and Rbfox3 for qPCR. please tell me if there are the others.

Thanks.