-using what buffer
-the protocol must be withe a reference (used befor in a scientific research )
This is a very vague question, give the details of your sample.
Method can be chosen depend upon the samples. there is four methods are Extraction, Precipitation, ultracentrifugation, and Chromatographic.
Please look at the following below links which may help you in your analysis:
http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/GE_ProteinSamplePreparationHandbook.pdf
https://file.scirp.org/pdf/ABB_2013043017032856.pdf
http://www.101bio.com/files/manual_P502.pdf
https://www.mobitec.com/cms/products/bio/03_proteomics/total_protein_extraction_for_animal_cultured_cells_and_tissues.html?pdf=SD-001_SN-002.pdf
Thanks
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