Is there some reason to not use 16S?

Most of the methods are focused on the whole 16S gene or using the hypervariable regions of the 16S.

Thank U very much.

Interesting. Thanks!

its nice suggestion by Stephen. Actually it all depends on the purpose and objectives like taxonomy, diagnosis, phyolgeny and so on.

In fact , Mr Sharma, the aim is to identify contaminant bacteria in sugar cane must during alcoholic fermentation.

Dear Ana,

I attach below then reference of an interesting recent article about 16S. I think it is important for your topic.

Bye, Olga

Taxonomic Classification of Bacterial 16S rRNA Genes Using Short Sequencing Reads: Evaluation of Effective Study Designs. 2013. PLOS ONE, 8(1): e53608.

Thank you very much Olga. I am sure it will be very helpful.

Thank you all for your interest. I am sure, Olga, that your suggestion will be very helpful.

Thanks!!