How have professors been able to transition complex ideas and concepts to a virtual environment?

As COVID-19 has forced academia to react instantly to the dynamics of teaching and learning, I am curious to find out how you have been able to transfer complex ideas that you would normally teach...

17 May 2020 8,254 16 View

Can I activate a mixed primary (mouse) glial culture, and then shake microglia off?

Our lab usually uses the shaking method to isolate primary mouse microglia from mixed glial cultures. Till now, I have been collecting microglia, plating them in isolation, and then activating...

17 November 2019 2,048 1 View

Has anyone had issues with extracting RNA from serum-grown bacterial cells?

In a side-by-side experiment, I got a normal amount of RNA from cells grown in chelexed BHI but very low yields from serum-grown cells. There was more cells in the serum culture, so low cell...

10 May 2018 2,167 3 View

My error correction term/speed of adjustment be -1.60529.?

I have performed VECM test with LLB and CPS as my independent variable and GDP as dependent variable. Can i write that In the absence of changes in LLB and CPS deviation of the model from long run...

18 August 2017 684 1 View

Do you have experience or protocols for cryofixation and sectioning of fetal/embryonic pigs?

14 December 2016 2,639 4 View

I have a mixed-design (2: condition X 2: pre-post) but due to a problem with data collection am unable to match pre-test with post-test data. Help?

I am in a bit of a bind with a project evaluating a classroom-based intervention. The design was supposed to be a mixed-design whereby I collected pre-post data from willing students linking...

05 December 2016 938 4 View

Hi, does anyone have an idea on how to purify a molecule with free thiol function without risking its oxidation?

I am interested in getting pure molecules with free thiol but the problem is that during the synthesis I always get a mixture of free thiols and disulphide bridge. After the purification on...

24 May 2016 1,699 1 View

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

14 February 2016 5,014 3 View

How long should I fix in Bouins for Masson's Trichrome?

02 August 2015 3,718 11 View

Processing for Cadaver Skin

25 August 2014 7,578 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View