I would like to ask you what is the best model to mimic human corpus luteum functions in vitro. I saw in literature that Granulosa Cells can be isolated from follicular liquid during oocyte withdraw for IVF. What would be the best protocol to differentiate these cells in granulosa-lutein cells? would it be reliable and scientifically accepted?
Good morning, I understand your interest for the best knowledge for high technology to FIV. It should be necessary to teach at the woman (before her hormonal stimulation and intervention) the knowledge of the natural signs of fertility, as cervical mucus for maximum days of fertility and basal temperature records to diagnose corpus luteum (less than 10 days of high level is insufficient CL, 10 days are sufficient CL, 16 days are maximum natural CL, 20 days diagnose a pregnancy.) If you are interested in more information about sympto-thermal method you can consult CaMen in Milan. All teachers of STM have cases of conception after unsuccessful FIV, but not all people conceive. It should be better to learn those signs before high technology. Regards
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