What is the best methods to transport growing MCF-7 cells in flask for 6 hours in ambient temperature?
Last week our team transported mcf-7 growing cells for 6 hours from another lab in another town by train, but after splitting we found that the cells are detached from flask and dying.
Any suggestion for best transport methods?
Here are the picture of the cells before transport. From the morphology, is there something wrong? It is our first time working with MCF-7
Thank you.
60-70% confluent cultures of cells in a flask can be transported or shipped without impacting their survival. Flasks need to be filled with culture medium completely without any air, the flask lid need to be closed tightly and sealed with parafilm.
Hi Andi,
You have to grow your cells until 30-50% confluency in T25 flask. You should full the flask with complete medium up to top (without any air). Close the flask with sterilized ( with70% EtOH and dried in A2 flow cabin) parafilm and sealed caps. Do not use ventilated caps. In this way, you can transport your cells at RT and they will be alive at least 3-7 days. For 6 hours, I believe that your cells will be fine. When the cells reach the final destination, let your cells settle for couple of hours in 37 CO2 incubator first. Then remove excessive medium and let the cells adapt for 1-2 days before splitting cells. I hope this can work for you. I sent once our cells to Egypt from Turkey in this way. It took more than 7 days and the cells were fine. I used this method many times without contamination.
Good luck
Cigdem
Thank you for the detail sharing of cell culture transport methods Professor Cigdem Ozen
Next week we are planning to transporting for the second times
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