Ceracystis paradoxa is isolated from suckers of pineapples used as planting materials. Ceratocystis paradoxa is an extremely destructive disease on pineapple. It can be managed in planting material by 'curing' the tops or slips before planting. Spread the planting material out in a single layer with the broken ends up and leave to dry for several days - they can be left like that for weeks if necessary provided they are kept in the shade. Alternatively, leave the tops and slips on the top of the parent plant to cure before collecting them for planting. Do not store planting material in heaps as this can lead to severe base rot, particularly in wet weather.Ceratocystis paradoxa is a pathogen of tropical crops notably palms like Borassus flabellifera, Cocos nucifera, Elaeis guineesis and crops like Carica papaya and Ipomea batatas .. Ceratocystis paradoxa is known by several names such as Thielaviopsis paradoxa, Chalara paradoxa. It belongs to the Ascomycetes group. Ceratocystis paradoxa is mainly soil borne. The pathogen can be spread from soils to coconut stems by splashing rain or irrigation water. Growth cracks on the coconut trunk, severe downpours, water stagnation, imbalances in nutrition, excess salinity, and plant stress can act as predisposing and aggravating factors . Its microspores can be stored in agar deeps with over layered 2% aqueous glyecrol layer at -20 degree C temperature.
Thanks for you answer Mr. Upadhyay. But with this low temperature this fungi produces the peritecia again in petri dishes?
After the conservation process when you cultured again in petri dishes with a proper media like carrot agar, C. paradoxa produce again the peritecia especially in aging colonies. But if your objective is store isolates for a later DNA extraction you don’t need the production of peritecia, you only need the production of biomass by the isolate to obtain good DNA. So the method recommended by Mr. Upadhyay is good and have been proved in many other species with good results.
Thank you for your answer Mr. Martinez. I really appreciate that
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