I would like to purify proteins from a seaweed hydrolyzate. The problem is the large amount of polysaccharides and that proteins are linked in it. So, which method can be used to isolate these proteins without denaturating it?
IF the protein of your interest is a glycoproteins, then worth using the lectin binding matrices like Concovalin A matrix or other glycoprotein purification matrices.
if the hydrolysate is a mixture of protein and sugars than concentrate the protein and pass it through gel filtration columns of small size exclusions.
you can read the following article for polyssaharide extraction and purification https://www.researchgate.net/publication/260110789_Characterization_of_the_flocculating_agent_from_the_spontaneously_flocculating_microalga_Chlorella_vulgaris_JSC-7
Article Characterization of the flocculating agent from the spontane...
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