Are there any sucrose gradient procedures available to you? I worked on Coxiella burnettii. we had to grow the bacteria in mouse spleen, then spin the spleen homogenates through sucrose gradients to isolate the infectious bacteria.

We have used Percoll gradient, but I havent thought about Sucrose gradient, we will perform some tests. Thankyou!.

...In addition to my inbox...perhaps you could substitute for sucrose as the gradient against which you can centrifuge. It has served me quite well...but this was for membrane protein isolation