I tested several conditions with fixing (4h PFA on ice), decalcification (24h EDTA, 4°C), sucrose (20%, 24h) and then OCT embedding. Still I get suboptimal sections. Any suggestions?
Thanks!
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
Dear Researchers, Recently, my research group and I have been working on understanding the formulation of axisymmetric elements. We began by studying 4-node and 8-node Axisymmetric-Harmonic...
12 July 2024 6,755 2 View
Je voudrais donner l'accès à la version auteur de mes articles référencés sur ResearchGate avec le lien HAL. Comment faire ? Où indiquer le lien HAL ? I would like to give access to the author...
27 May 2024 2,668 3 View
Would it be possible to hydrogenate titanium dioxide to titanium metal with a platinum catalyst in ammonia, ethylamine or ethylenediamine?
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I would like to export several search result lists from different databases (ProQuest, Scopus, WoS, Ebsco) and use them for a bibliographic analysis. In this context, I would also like to do the...
27 April 2024 3,961 1 View
I've 2 profiles unfortunately, one with my office-adress and one with my institutional address. The first one I maintain on an ongoing basis with my publications
21 April 2024 5,794 3 View
Hello! I am currently working on a study using the SOFC model with unresolved electrolyte. I am getting a very low current. If I lower my applied voltage in the cathode terminal, the calculated...
07 March 2024 6,083 0 View
The procedures of Justus Liebig Annalen der Chemie 1914 do not lead to the desired product.
25 February 2024 6,511 4 View
For those in the field of environmental / climate diplomacy, are there any gaps in research in this area of study that you think should be further investigated? I'm currently starting my masters...
15 February 2024 1,222 2 View
Hello! I am currently doing my MS Thesis. I am having trouble in creating the electrolyte in ANSYS. From the manual, it should be a pair of shadow walls. However, since both the anode and cathode...
28 January 2024 1,690 0 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
whenever I take cryostat sections of rat brain, many holes are distorting the normal morphology(image attached). what may be the reason? protocol which we follow: * Perfuse the brain in PBS and...
15 June 2024 1,282 1 View