I am trying to optimize gene (click beetle luciferase) expression in Listeria (gram+), how and what is the best method to do codon optimization, finding out rare codon etc. is there any good software ?
Dear Sadeeq Ur Rehman: We have published an article on this. Please see for details: The full article is available on my RG-link, page 2. Good luck
Article: Over expression of a synthetic gene encoding interferon lambda using relative synonymous Codon usage bias in Escherichia coli.
Hashaam Akhtar · Samar Akhtar · Syed Umer Jan · Azka Khan · Najamus-Sahar Sadaf Zaidi · Ishtiaq Qadri
[Show abstract]
Pakistan journal of pharmaceutical sciences 11/2013; 26(6):1181-8
Thanks Ishiaq sab and happy to see your good contribution in science. I guess i had met you during a conference at LUMS in Pakistan.
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