The best method used for the criteria of measuring and evaluation of semen in rats
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
How is pharmacogenomics transforming personalized medicine, and what are the current challenges in its implementation?
05 July 2024 5,082 2 View
Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...
02 July 2024 5,502 4 View
I am preparing polycrystalline diamond (PCD) with tape casting. The powder is 8~12 um. After drying of tapes, I place several tapes in Ta cup without warm-pressing. Next, debinding is carried...
23 June 2024 7,758 2 View
Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....
19 May 2024 3,164 2 View
https://docs.google.com/forms/d/e/1FAIpQLSe4Ht6uSWs_IOArQZyHYtfYD7aEFJBxFnIgJ46yPQ_k7rbDjg/viewform?usp=sf_link
11 April 2024 3,417 0 View
mutations play a critical role in driving the adaptation of microbial populations to diverse environments, allowing them to thrive in a wide range of ecological niches.
04 April 2024 8,855 3 View
In my opinion, I could say: Benefits: Accelerated Drug Discovery Cost Reduction Optimized Clinical Trials Challenges: Dealing with big data Over-fitting and Generalization Human Expertise and...
01 April 2024 5,706 4 View
Hello to all possible researchers who have a developed research idea in the field of social psychology, specifically the behavior of individuals in groups
29 March 2024 3,809 6 View
for example dependent variable is daily stock returns and independent variables are company characteristics such as firm size, leverage etc.
21 March 2024 362 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View