The best method used for the criteria of measuring and evaluation of semen in rats
I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...
01 March 2021 395 3 View
HDPE samples were aged at higher temperatures in the presence of water and CO2 than the TGA test was performed in Nitrogen? Weight gain is seen in the curve? What could be the possible reason for...
16 January 2021 5,644 2 View
As it has known the Dirichlet boundary condition is suitable for no-slip wall boundary for the stream function in COMSOL. I need to ask if I have an inlet and outlet I need to look for another...
02 January 2021 6,255 4 View
Greetings; I've recently published a preprint for a COVID treatment RCT, which appeared under "Dental" specialty instead of "Infectious diseases (ID)". This is an issue as it doesn't appear under...
14 December 2020 6,614 4 View
I have conducted a survey which includes two groups (1-Normal people, 2-Deaf and Dumb), and the questionnaire had a category of questions with 4 different options and to answer only tick one. I...
01 December 2020 1,147 3 View
I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...
29 November 2020 3,165 3 View
I used this method to make concentrations, by using distilled water to make concentration like 25mg/ml 25mg of extract in 1ml of distilled water and so on ....
29 November 2020 2,933 4 View
Hi Folks I am working on my thesis for my Master's degree completion. I have produced a survey on the above topic. I need some expert's opinions and recommendations for the questions of the...
02 November 2020 4,870 3 View
The question also includes the assurance of safety in the self-driving cars and the expected cost of selling. And what kind of quantum model will be more suitable in terms of physical realization?
29 October 2020 5,352 3 View
The introduction of time-fractional derivatives in the Navier-Stokes equations goes back to Lions and was recently extensively studied by many authors. When motivating such problems authors tend...
26 October 2020 9,642 4 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...
25 February 2021 7,698 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View
I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...
09 February 2021 9,480 1 View