I want to assess of enzymes activity of 500 isolates of Trichoderma.

I want to identify best isolates of Trichoderma and then I will use it as a biological agent against some of most important plant disease fungi such as Macrophomina phaseolina and Sclerotinia sclerotiorum. After identification of the best isolates in enzymes producing, I can test them on plant.I have read in many articles that glucanases and chitinases play a role in the biological control of Trichoderma isolates,but I need to sure that which enzymes are important and which enzymes are useful and applied in control biologic.1. Which enzymes are important in biological control of plant diseases?For example: If Glucanases and Chitinases are important so that:2. What is the best method to assess of these enzymes activity of Trichoderma isolates?