I am currently trying to extract Genomic DNA from various soil samples. Usually, I pick bulk soil from which an aliquot is taken for DNA extraction. Most protocols I have seen use between 0.2 to 1g of the soil sample for DNA extraction and analysis. But I am thinking that using only 0.5g, for instance, from a bulk sample of 10-20 gram is not reprentative enough.
Could someone help me here. Is there a method for homogenizing the bulk sample before taking an aliquot? or is it proper to homogenize the bulk sample by mixing with sterile de iodixed water or some sort of buffer and then use an aliquot of the soil/water mixture for the extration?
What kit is best for DNA extraction from soil?
Thank you in anticipation of your response
I do not know what you mean by best. I think is the method of extraction, to a large extent, depends on the use to which you will put the DNA extract. See the link attached, please. Article Methods of microbial DNA extraction from soil by PCR amplification
Hello
I have used various methods based on SDS-proteinase K and they were OK. But when I used this kit: Genomic DNA from soil from Mecherey Nagel company (https://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/Genomic%20DNA/UM_gDNASoil.pdf) the purity of DNA was much higher. I do recommend it.
Good luck
Maurice and Ali Thank you very much. I find your responses very useful.
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