I have a pure culture of diatom. Can anybody explain a simple method of diatom DNA isolation?
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Enhancing parental satisfaction through the comprehensive debriefing of fetal anomalies using 3D models- this is my research topic. I kindly request to suggest some questions that can be asked to...
06 July 2024 8,032 0 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View
BET analysis
27 June 2024 6,964 1 View
Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...
25 June 2024 9,151 6 View
pI of my protein is 4.1. I am using Ni coumn to purify it . Which binding buffers will be good? Even the pI is low can it still bind to nucleic acid? How do I know?
04 May 2024 8,535 2 View
I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...
25 April 2024 9,798 0 View
I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...
07 April 2024 3,079 1 View
Dear Networks, Seeking user-friendly software for memristor design in Neuromorphic Computing. Suggestions with links appreciated! Let's simplify the process and collaborate on efficient...
13 March 2024 5,558 1 View
I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...
18 January 2024 6,800 1 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...
18 July 2024 7,258 3 View
can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...
17 July 2024 3,687 11 View
How use this chamber and standarize the data
17 July 2024 1,844 2 View
In saw this creature during a cable laying operation at 20km off the coast of Timor Leste on the timor sea at a depth of 1200 m.
14 July 2024 4,715 1 View
Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...
12 July 2024 159 2 View
I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...
08 July 2024 3,342 5 View
What could be the reason for mixotrophs to be yellow too instead of green? Thank you
08 July 2024 594 2 View