I have tried Pikovaskya medium, but without any results? I need a method in liquid medium
use the qualitative methode : solubilisation of phosphate on National Botanical Research Institute Phosphate medium (NBRIP) with 0.5% of tricalcium phosphate (Ca3(PO4)2). and 0.025g/l of BPB (Bromopenol Blue) to visualise more the zone of solubilization. and after calculation of solubilization index, you choose the best bacteria for quantitative test at liquid medium.
use the qualitative methode : solubilisation of phosphate on National Botanical Research Institute Phosphate medium (NBRIP) with 0.5% of tricalcium phosphate (Ca3(PO4)2). and 0.025g/l of BPB (Bromopenol Blue) to visualise more the zone of solubilization. and after calculation of solubilization index, you choose the best bacteria for quantitative test at liquid medium.
There is a nice paper comparing several medium and growth conditions for this solubilisation of phosphate test: Nautiyal CS/FEMS Microbiology Letters 170 (1999) 265-270.
Hi Hafida,
There are different type of media used to reveal the activity "phosphate solubilization": Sperber Medium, PVK (Pikovaskya), GES and so on. However, the best one the I used myself is NBRIP as M. Ettadili said. Following the link below, you can find an article that explain the steps which you have follow to isolate and screen the most performance PSB.
Good reading
Hi Zakaria,
Thank you for your answer, I see that there is no link .
In general , Does the period of incubation affect the screening of phospahte solubilzation ?
THank you Hamza, It is a good idea to use the bromophenol blue.
Thank you Carmen. I have already read it
Excuse me Hafida, this is the link (https://www.researchgate.net/profile/Rfaki_Abderrazak2).
Of course, the time of the incubation has an influence on phosphate solubilization. It's depend of the your isolats and also of the media used to reveal this function. you can find many articles which are using different ranges of time. in general, between 5 days to 10. However, there are others who remain the dishes in incubation for long time in order to determine the evolution of the phosphate solubilization index with time. And this evolution can help you to screen the most efficient strains.
Good luck
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