I want to prepare protein powder from different sources. what is the best extraction technique that gives maximum yield, edible and also possible for pilot scale production?
You should try to refine your quest. Edible proteins may possibly differ from from their raw material protein sources; such as gelatin is obtained from collagen. Methods for extraction/purification of proteins are typically strongly protein and raw material dependent ― for instance; the following RG discussions may possibly be found of some help while considering either elastin, keratin, and collagen, respectively:
https://www.researchgate.net/post/What_is_the_appropriate_solvent_to_dissolve_powdered_cartilage https://www.researchgate.net/post/Protein_extraction_from_chicken_feathers https://www.researchgate.net/post/Alternative_methods_to_precipitate_collagen_proteins_with_neutral_salts
Read the following title:
Mecham, R.P., 2008. Methods in elastic tissue biology: elastin isolation and purification. Methods, 45(1), pp.32-41.
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