I'm working on confocal imaging of attine ants fungus gardens, which are sponge-like fungal structures containing fragmented plant material and an associated bacterial microbiota. To look into the fungus gardens structure, we are attempting to embedd the structure and to use the microtome to get longitudinal sections.
Our first attempt using Leica Historesin did not render good results for FISH. Does anyone know a better method that could render good quality FISH results?
Thanks
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122835/
https://academic.oup.com/biohorizons/article/3/1/85/228880
Article Evaluation of fluorescence in situ hybridisation (FISH) for ...
https://www.microscopyu.com/applications/fluorescence-in-situ-hybridization-fish/fluorescence-in-situ-hybridization
There are several methods that can be used for solid embedding of fungal specimens to allow fluorescence in situ hybridization (FISH) and confocal imaging. Some of the most common methods include:
Each of these methods has its own advantages and disadvantages, and the best method for solid embedding of fungal specimens will depend on the specific requirements of the experiment and the characteristics of the specimens. It is important to carefully consider the specific needs of the experiment and the properties of the specimens when selecting a method for solid embedding.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column