I have read article for preparation of amyloid beta 1-42 oligomer by HFIP and NH4OH dissolving method. plz suggest me the best method.
There are several well established procedures. What is the form of amyloid beta 1-42 protein that you have ? TFA salt ?
The amyloid beta 1-42 is in solid white powder form.
Appearance (Color) =White
Appearance (Form)= Powder
Trifluoroacetate Content = _ 65 %
Purity (HPLC) = >_ 95 %
plz give suggestion.
Oligomer prep
48 hours prior to use:
1. Dissolve 1 aliquot of Aβ (79-80 microgram of peptide) in 50 µl HFIP
2. Add 175µl sterile H2O
3. Poke a couple of holes on the Eppendorf tube cap (using a needle)
4. Place a stir bar in the tube and place the Eppendorf tube on a stir plate for 48 hours (at RT).
5. Resulting peptide should be close to 100 microM. Check final concentration with BCA or Bradford.
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