Hello, i am looking for a method for transient or stable transfection of a lepidoptera embryonal cell line we have in our lab. I want to avoid viral transfection like lenti- or baculoviral systems due to cytopathic effects because i want to use the transfected cells for live-cell imaging. I do have the sequence of the protein i want to transfect in a pcDNA3 vector or purified from HEK cells so i could either transfect the DNA with a suitable vector or the Protein directly, any advice ?

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