Jan Bröckel

4 Questions 2 Answers 0 Followers

Questions related from Jan Bröckel

What is the best method for non-viral transfection of insect cell lines ?

Hello, i am looking for a method for transient or stable transfection of a lepidoptera embryonal cell line we have in our lab. I want to avoid viral transfection like lenti- or baculoviral systems...

17 April 2023 169 0 View

Are there any methods of transfection/transduction of insect cell cultures for live cell imaging ?

Hey, i want to measure cAMP in insect (cockroach/hawkmoth) cell cultures, either in primary cell cultures of neurons (preferred) or in stable embryonic cell cultures. I have plasmids for a cAMP...

28 March 2023 5,111 2 View

How can i find a specific gene in a genome ?

I am looking for the gene of a specific protein in a sequenced genome of Manduca sexta. The Genome (JHU_Msex_v1.0) should be available online and the protein i am looking for is protein kinas A...

26 April 2022 4,676 1 View

How can i suspend Alginate particles in aqueous media for zeta potential meausurement ?

I obtained Alginate particles (microparticles) and want to meausure zeta potential, therefore i need a suspension of particles. Yet, the particles only dissolve completely in aqueous media. In...

24 August 2020 9,997 4 View