lease suggest best protocol for getting cyanobacterial cell free enzymatic extract, can anybody please specify the exact amount of chemical needed ( KPP,EDTA,TRITON X,PVP,AND PMSF ) for making final extraction buffer.
Dear Niharika Sahu many thanks for posting this interesting technical question. As an inorganic chemist I'm absolutely no expert in this field. However, I just came across the following potentially useful articles which might help you in your analysis:
An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria
Article An easy and efficient permeabilization protocol for in vivo ...
This paper has been posted by the authors as public full text on RG. Thus it can be freely downloaded as pdf file.
Also please go through the following article which is also freely available as public full text:
Chemical lysis of cyanobacteria
Article Chemical lysis of cyanobacteria
I hope this helps. Good luck with you research and best wishes!