What is the best method for determination of keratinase activity of culture broth after degradation of feathers by keratinolytic bacteria?

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Masashi Yoda

Hello,

You can use casein as a substrate of keratinase and quantify the amount of glycin with ninhydrin reaction method. There are numbers of ningydrin reaction test kit for quantification of amino acids, and generally they measures the absorbance of 570 nm.

Eriston Gomes

Dear colleague,

have you used this suggested technique to determine keratinolytic activity?