MEA or MYEA media are used a lot for fungal metabolites; but you will need a to costumize the media depending on what type of fungal metabolites you are looking for; Hope it helps.
Here you step some of the most used in medical mycology media:
Czapek Dox Agar for cultivation fungi Aspergillus spp and Penicillium spp
Modified Dixon agar (mDixon) for growing Malssezia spp
MEA for yeasts and mycelial fungi. There are several formulations including malt extract agar supplemented with peptones, maltose and / or dextrin and / or glycerol
PDA appropriate to stimulate the formation of conidia in the preparation of inoculum of mycelial fungi and stimulation of red pigments in Trichophyton rubrum, Microsporum audouinii pink and yellow Microsoporum canis.
SDA (Sabouraud Dextrose Agar Emmons modified): is a standard means of stimulating sporulation and fungal conservation. The change in pH and glucose concentration promotes sporulation.
Oatmeal agar (OA): recommended for Acremonium, Cladosporium, Fusarium, Paecilomyces, Scopulariopsis and for obtaining teleophorm associated forms.
Corn meal agar with Tween 80: Candida species. The Tween is added to show the formation of chlamydospores.
It milk agar, glucose and bromocresol purple is used to differentiate .: Trichophyton rubrum and T. mentagrophytes.
Agar modified Leonian: indicated to stimulate sporulation in strains that do not sporulate or have aberrant structures.
Potato carrot agar (PCA), to stimulate sporulation of mycelial fungi, very suitable for dematiaceous.
CHROMagar Candida contains various chromogenic enzyme substrates bonded compounds. It is to evaluate the colonies at 48 hours.
Hope it helps
Dear All, I don't think answers are definite. Suitability of media mostly depends on fungal genera & species in question, and target secondary metabolites. For example, Yeast extract sucrose (YES) agar and Czapek yeast autolysate (CYA) agar are good for assaying a range of secondary metabolites from Aspergillus species while autoclaved corn or rice are very useful for screening secondary metabolite production in Fusarium spp.
Zulqarnain: Can you kindly send me a request on and I attach you the papers on secondary metabolite screening in Aspergillus and Fusarium species. It's difficult for me here attaching more than one paper; files are heavy. Other interested person can also make requests. Thank you.
Dear Chibundu thank you I followed your profile and could you please send me those papers on my email [email protected]?
Bhavin if i am interested in Antibacterial, antifungal or nematicidal compounds then?
I've always found an enormous difference in the amount of the secondary metabolites produced by fungi on different medium, but not really in the structural core of the molecules. Secondary metabolites are produced in a family, quite related each other. So in relation to the chosen medium (solid or liquid; stationary or shaken), its composition (I found the major influence in the percentage of sugar), light or dark, time of the cultures before extraction, you can get different quantities of the metabolites, but little differences in the chemical structures: anyway just a chemical variation as an acetyl group or a methyl group or an oxygen inserted or a double bond or a cyclization of a lateral chain will result in a very different compound also considering the biological activity. There's no a recipe valid for all fungi. Just try to cultivate them and see. In our experience we use Roux flasks with agar medium and we extract the fungus cultures 14 d after inoculum leaving organic solvent O/N before evaporating it. Best wishes.
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