How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

Can you help me?

I am looking for information as to whether some insects (or spiders?) similar in pattern of colouration (red anteriorly, blackish posteriorly, white transverse bands) to some Cleridae (Clerus,...

09 February 2020 4,416 3 View

Does somebody know about protocol of retroviruses and adeno-assosiated viruses?

Hello! I have a lot of practice with lentiviruses (shRNA, knock-in, CRISPR, etc.). And now I need to transduce human and mouse cells by retroviruses (knock-in). I recieved plasmid pQCXIP and in...

11 January 2020 1,245 3 View

Variation partitioning - Is there a all -encompassing function/package in R?

I am a bit frustrated looking for a competent function doing variation partitioning (e.g. on linear model output) it should be able to tick the following boxes A) calculate all overlaps and "pure"...

04 December 2019 9,273 3 View

Is there any problem with magnetite nanoparticles to be observed in a FESEM system?

I ask this because I had a problem with a FESEM FEI SCIOS system. According to the technician he made observations of my sample TiO2/MAGNETITE. After many observations and session of my samples...

25 November 2019 3,024 4 View

Has anyone had problems quantifying DNA using Qubit?

I use Qubit to quantify my DNA material, and I have never had quantification problems. However a few weeks ago my lab bought a new Qubit reactive which has a different color (slightly pink) in...

23 September 2019 3,943 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View