Dear colleagues, I need specialists’ suggestions who deal with the extraction of polyphenols.
I am wondering whether there is a green and cost-efficient extraction technique of ferulic acid from wheat and rye bran?
Based on my results and the data obtained by other researchers I know that ferulic acid is the main phenolic acids representative found in cereals. However, this hydroxycinnamic acid derivative is extremely integrated into cell-walls of plant material and classified as non-extractable polyphenol. The recovery of ferulic acid makes up 1%.
I did several experiments with different bran samples using conventional extractions; however, the results leave something to be desired.
I also conducted hydrolysis of my samples with NaOH and HCl, the recovery is much better, though this approach led to the destruction of acid, base, and thermolabile compounds.
Thank you very much for your valuable suggestions and comments on this matter!
Yours sincerely,
Vitali
Dear Vitalijs Radenkovs
According to my experience, The use of Ultrasound-assisted extraction as an extraction method and the methanol + KOH (5%) as an extracting solvent may give a satisfy result.
All the best.
Dear Mac!
Thank you very much for your reply. Much appreciated!
These samples have already been analyzed by LC-MS/MS. The recovery of ferulic acid from wheat and rye bran using EtOH, MeOH as solvents combining with ultrasound-assisted extraction is very low.
The question is whether there are other approaches to the effective extraction of this compound?
Yours sincerely,
Vitali
Dear Carole Tranchant!
Thank you so much for your answer and for valuable information!
I noticed that you did alkaline and acid hydrolysis of your samples to release bound forms of phenolics.
In my experiment, I also have used very mild alkaline hydrolysis for 4 hours. This extraction is able to release bound ferulic acid, however, at the same time, I observed a decrease in the concentration of other compounds that were well extracted using MeOH or EtOH without treatment.
Yours sincerely,
Vitali
Dear Carole!
Thank you for your interest!
Definitely, I will inform you once these data are published!
Good luck,
Vitali
Dear Mac!
Thank you very much for the information provided! I really appreciate your contribution.
Yours sincerely,
Vitali
Dear Vitalijs Radenkovs, please find attached the results of a literature search on this topic. Unfortunately, the references describing ultrasound-assisted extraction are written in Chinese. Nevertheless, I hope it helps.
Dear Frank T. Edelmann,
Many thanks for your efforts in providing these references. Much appreciated!
Yours sincerely,
Vitali
Dear Vitalijs Radenkovs
According to my experience, The use of Ultrasound-assisted extraction as an extraction method and the methanol + KOH (5%) as an extracting solvent may give a satisfy result.
All the best.
Dear Yasser Fakri Mustafa,
Thank you so much for the suggestion. I would appreciate if you could specify the concentration of methanol used in your experiments and the duration of ultrasound-assisted extraction.
Thanks,
Yours sincerely,
Vitali
you can read this paper
Article Contents, profiles and bioactive properties of free and boun...
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