Dear Vitalijs Radenkovs

According to my experience, The use of Ultrasound-assisted extraction as an extraction method and the methanol + KOH (5%) as an extracting solvent may give a satisfy result.

All the best.

Dear Vitalijs Radenkovs

I think you can use HPLC (High Performance Liquid Chromatography) and mass spectrometry to identify ferulic acid in your extract.

Dear Mac!

Thank you very much for your reply. Much appreciated!

These samples have already been analyzed by LC-MS/MS. The recovery of ferulic acid from wheat and rye bran using EtOH, MeOH as solvents combining with ultrasound-assisted extraction is very low.

The question is whether there are other approaches to the effective extraction of this compound?

Yours sincerely,

Vitali

Dear Carole Tranchant!

Thank you so much for your answer and for valuable information!

I noticed that you did alkaline and acid hydrolysis of your samples to release bound forms of phenolics.

In my experiment, I also have used very mild alkaline hydrolysis for 4 hours. This extraction is able to release bound ferulic acid, however, at the same time, I observed a decrease in the concentration of other compounds that were well extracted using MeOH or EtOH without treatment.

Yours sincerely,

Vitali

Dear Carole!

Thank you for your interest!

Definitely, I will inform you once these data are published!

Good luck,

Vitali

Vitalijs Radenkovs

Dear Vitalijs Radenkovs

You're most welcome. Kindly follow the link bellow which might be helpful.

Article Advances in combined enzymatic extraction of ferulic acid fr...

Dear Mac!

Thank you very much for the information provided! I really appreciate your contribution.

Yours sincerely,

Vitali

Dear Vitalijs Radenkovs, please find attached the results of a literature search on this topic. Unfortunately, the references describing ultrasound-assisted extraction are written in Chinese. Nevertheless, I hope it helps.

Dear Frank T. Edelmann,

Many thanks for your efforts in providing these references. Much appreciated!

Yours sincerely,

Vitali

Dear Vitalijs Radenkovs

According to my experience, The use of Ultrasound-assisted extraction as an extraction method and the methanol + KOH (5%) as an extracting solvent may give a satisfy result.

All the best.

Dear Yasser Fakri Mustafa,

Thank you so much for the suggestion. I would appreciate if you could specify the concentration of methanol used in your experiments and the duration of ultrasound-assisted extraction.

Thanks,

Yours sincerely,

Vitali

you can read this paper

Article Contents, profiles and bioactive properties of free and boun...