Can anyone advise me on the best dopaminergic cell lines? I am particularly interested in finding out more about RCSN-3. What are the pros and cons? Are they difficult/expensive to culture? Would anyone recommend using another cell line over RCSN-3?
Hi Kathryn! LUHMES cells are the best for in vitro experiments in a PD context, since they "differentiate" into typical dopaminergic neurons, that are sensitive to iron, and able to undergo ferroptosis. You can buy them on ATCC I guess. Good luck!
Hi Kathryn, I agree with Vincent that the LUHMES are a human in vitro model. LUHMES aren't impossible to grow or differentiate, but they aren't the easiest cells to work with. The proper differentiation of these cells can be a bit costlier than just having a cell line, but they ARE human and that helps. I have not worked with the RCSN-3 cell line, but they seem very attractive and could be a better alternative than the N27 cell line that most use. Depending on your study and if you need a particular species (MN9D cells are mouse) for conditioned media experiments or such then there are definitely some to choose from. Hope this helps and good luck!
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