Hello

Try with anti-DCX antibody :

https://www.mybiosource.com/polyclonal-cow-dog-human-mouse-pig-rat-antibody/doublecortin-dcx/422562

Good Luck

Hi Amanda,

https://www.scbt.com/scbt/product/doublecortin-antibody-c-18

Doublecortin (C-18) has been discontinued and replaced by Doublecortin (E-6): sc-271390. This new monoclonal antibody is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

This other from Abcam (Anti-Doublecortin antibody (ab18723) is quite good, although expensive as well.

https://www.abcam.com/doublecortin-antibody-ab18723.html

Cheers,

Santa Cruz itself noted on its webpage for sc-8066 DCX antibody that its production has been discontinued, and suggested to use Doublecortin (E-6): sc-271390. Check the details here:

https://www.scbt.com/scbt/product/doublecortin-antibody-e-6

Also, if you insist or comfortable to use Santa Cruz IHC antibodies, they suggest a list of these E-6 antibodies for specific uses, check and see which suits your purpose better:

https://www.scbt.com/scbt/product/doublecortin-antibody-c-18?productCanUrl=doublecortin-antibody-c-18&_requestid=17435048

Thanks everyone, I have a sample of the new Santa Cruz antibody on the way!

I agree with Bahman Sadeghi , The antibody (Doublecortin (E-6): sc-271390 Santa Cruz) works well.

I also attached my IHC image.

Wulaer Bolati Oh wow that looks great!

I would propose

Anti-Doublecortin antibody (cat# ab18723) from Abcam

Wulaer Bolati , Do you have to do antigen retrieval to get the staining you showed. We tried it without antigen retrieval and staining is very weak. What dilution did you use? Thank you so much!

Hey! Just to add to the great answers here:

My go to DCX:

Doublecortin-- shown in purple (Millipore AB2253)-- Guinea Pig--- (1:1000)

I hope this helps!

E

Agnes Luo Sorry for the late reply. Yes, I use citrate and heat-induced antigen retrieval, the dilution is 1:200.

Wulaer Bolati , Thank you! We will give it a try.

Elliot Glotfelty , awesome images. Yes, we have this Guinea Pig one and it works really well but very expensive. We are trying to find an alternative one. Thanks for your answer!

@WulaerBolati Can i ask how (room temp, 4°c, etc) and for long you incubated the ab for? And what were the conditions of your Antigen Retrieval?

Amanda Kalliopi Emma Hornsby Hey, I translated the protocol into English for you, check the protocol in the attached file. Don't hesitate to ask me any question.