What is the best criteria to select Arabidopsis transgenic lines for phenotype analysis? other than expression level (qRT pcr)?

13 September 2023 1 7K Report

Because expression has not always linear relationship with phenotype

I'm in favor of using several homozygous lines that appear to have single insertions and a range of gene expression levels. That way you can see if there is a trend for expression level and phenotype of interest.

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