Good day All
I am busy cleaning up the microalgae strain Haematococcus pluvialis isolated from my bird bath outside. I have been battling to get rid of the Paraphysoderma sedebokerense (fungal parasite) on this strain for a very long time. Is there perhaps someone in this community with more experience who can advise me on which specific antibiotics and / or fungicides would be most effective for cleaning H. pluvialis? Your help will be much appreciated.
Hello, Linki!
I usually get axenic strains after agar plate cultivation. Do you know how to do this procedure? Have you already tried this method?
All the best,
Thais
Hi Thais, thank you for answering. I have streaked too many plates to count. I look for a colony under the microscope, transfer it but a few days after, I have contamination again. I suspect I am transferring the fungi spores too. H. pluvialis is quite a slow grower. I have good colonies after a week but I suspect that they probably get contaminated at day 2 - 4. The fungi grows and spreads quicker than I can get colonies. I know I can buy a clean strain but I really want to get this right and clean my own. I can see it is a good strain with large cells and very well acclimatized already.
From what I have read cultivation on an acidic medium totally prevents the infection.
Check: Article Acidic cultivation of Haematococcus pluvialis for improved a...
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