Simply you need to perform, quantitative analysis of mRNA that you have silenced against siRNA. not only the the %age on mRNA reduction but also the corresponding protein in cell/tissue lysates as well as in circulation. and compare with normal/controls (untransfected group).
order Silencer Select siRNA, use RNAiMax transfection reagent, at 24h post-transfection measure mRNA knockdown by qRT-PCR with corresponding TaqMan assay