Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Mie scattering of a Gaussian with curved wavefront by a sphere?

Can anyone please suggest some references? I would like to simulate scattering of a fundamental mode of a fiber by a microsphere. I am only familiar with a plane wave case, but in case of a curved...

01 January 2021 1,440 3 View

Blood and tissue DNeasy mini kit 260/230 contamination?

I've been trying to extract DNA from rat liver tissue using Blood and tissue DNeasy mini kit. I followed handbook procedure and all of my samples had low 260/230 (

21 December 2020 7,100 3 View

Student's t-test or DeSeq2 for RNA-Seq data analysis?

Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...

28 November 2020 5,977 3 View

Cre-loxp system does not work (lines #017455 and #017320, JAX). Why?

We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...

16 November 2020 8,701 1 View

Why after cloning recombinant pET in E. coli DH5a I get a very low plasmid yield after extraction?

I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....

13 September 2020 4,421 3 View

Good markers to distinguish D1R/D2R striatal medium spiny neurons?

Hi! I want to distinguish D1R and D2R subpopulations of medium spiny neurons in striatum in immunofluorescence. Most of the papers I found use transgenic EGFP mice targeting this receptors, but I...

31 August 2020 3,863 2 View

does anyone have the user manual of the geostrain program?

Hello, I am looking for the user manual of the geostrain software, I have downloaded the software but the user manual is not found. I will be very grateful for your help Cheers

14 June 2020 4,169 1 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View