I need to measure ROS from isolated mitochondria. I am confused if I should measure luminescence or fluorescence for this. Can anyone recommend me the best approach for this?
The protocol which consists in measuring H2O2 production using Amplex Red and HRP is well established and validated. It is a good method to assess mitochondrial ROS production even if it does not allow the direct assessment of superoxide production. It is well explained in the publication recommended by A Timimi Zahra why one can be confident when this method is used.