Iru Paudel

3 Questions 5 Answers 0 Followers

Questions related from Iru Paudel

What concentration of mitotracker green staining do you use for HeLa cells?

I have been trying to optimize Mito tracker green FM staining for HeLa cells. I have used different concentration ranging from 50nm to 2 um (50nm, 100nm, 200nm, 500nm, 1um, 2um) for 25-30 mins. I...

21 February 2017 8,370 3 View

Focus position not found while using olympus fluoview microscope. Has anyone faced the same issue?

Hello everyone, I have just started using Olympus fluoview for confocal microscopy and I don't know much about microscopy. I plate my cells (HeLa) in 4 welled Chambers slide. Stain the cells with...

14 October 2016 5,287 5 View

What is the best approach to measure reactive oxygen species from isolated mitochondria?

I need to measure ROS from isolated mitochondria. I am confused if I should measure luminescence or fluorescence for this. Can anyone recommend me the best approach for this?

01 January 1970 1,527 3 View