A loopful of the plant extract will streak onto a sterile Nutrient agar (for bacterial contamination) and Sabouraud’s agar plates (for fungal contamination). These media plates will be incubated at room temperature for 48h, following which they will be examined for any bacterial or fungal contaminations respectively.

Cheers

Thanks you so much

Dear , Anashuya Biswas-Raha.

You must take a sample from the plant extract and added it to Petri dishes containing suitable media for bacteria or fungi under laminar air flow conditions , and then incubate the plates under 25 - 30 C in incubator for 2 - 3 days. Then examine the plates , if you see no bacterial or fungal growth , that indicates the plant extract is not contaminated .

With best regards

 Hi, Please take your extract serially dilute using sterile water and spread it on PDA or NA. If there is any emergence of bacteria or fungus  indicates your extract is contaminated.

Regards

Dear Anashuya

I prefer to pass the whole plant extracts  through a millipore  0.22 micro filters,  so you will have sterilized plant extract from  any fungal  contaminants . If you want to check entierly ,you can use PDA medium after using millipore filter>

You can use filtrate with 0.22 micron filter. 

If you want to check if your extract is contaminated by fungi and be sure that it is not a bacterial contamination, spread it on medium for fungi (PDA or Saboraud's agar) supplemented with antobiotic (to avoid bacterial growth) such as chloramphenicol, ampycillin or oxytetracycyline.

regards

Really i agreed with dear Oadi Manty ,First you can filter extract with either 0.45 or 0.22 micron millipore , then if want confirmation, you can check with PDA or any medium for Fungi

Hi Anashuya

See some references 1

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQFgguMAI&url=http%3A%2F%2Fwww.level.com.tw%2Fhtml%2Fezcatfiles%2Fvipweb20%2Fimg%2Fimg%2F20297%2Fcontamination-COR.pdf&usg=AOvVaw0OQW5zqBq5h_9jN1aSQLGS

2

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQFgg3MAM&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fj.1365-2672.2010.04776.x%2Fpdf&usg=AOvVaw2oGm3H2hf6clqXJ1ZP4bQF

3

https://books.google.iq/books?id=hgXtCAAAQBAJ&pg=PA619&lpg=PA619&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=rGNpG1p89-&sig=J1rWDJ8l5b4BGO9h5jbz3nddYQw&hl=en&sa=X&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQ6AEIPzAE

4

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQFghLMAY&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC3505157%2F&usg=AOvVaw3uPjUQv18NhkXPloAoZytU

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&cad=rja&uact=8&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQFghUMAc&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC5209813%2F&usg=AOvVaw0A7g_2cFQVV4uvVkmVq65q

6

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQFghdMAg&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC3912047%2F&usg=AOvVaw21SH7N-gUsJ2sZ3pJ1YUW7

7

https://books.google.iq/books?id=mRwPDQAAQBAJ&pg=PA558&lpg=PA558&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=RaZNm9n6Gn&sig=dX3kHIfWop5YIlIVyO0HIZMlwk0&hl=en&sa=X&ved=0ahUKEwi0rIH8-ZDYAhUIalAKHYEEDwoQ6AEIZjAJ

8

https://books.google.iq/books?id=AST-CAAAQBAJ&pg=PA240&lpg=PA240&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=Zf9mpInwAX&sig=zK33cjUGo6tYtdbpTOq_I_DZZ4Q&hl=en&sa=X&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChDoAQgmMAA

9

https://books.google.iq/books?id=sSHIlWSOiroC&pg=PA914&lpg=PA914&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=lhDKPSmSLg&sig=prHXwbF3T5zPGSpiBIZyZ_8Pciw&hl=en&sa=X&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChDoAQgoMAE

10

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCCowAg&url=http%3A%2F%2Fwww.scielo.cl%2Fpdf%2Fchiljar%2Fv71n2%2Fat08.pdf&usg=AOvVaw3-bZCFGVy6WYI_ilDzo6mM

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCDEwAw&url=http%3A%2F%2Fwww.nature.com%2Fismej%2Fjournal%2Fv8%2Fn2%2Ffull%2Fismej2013149a.html&usg=AOvVaw3qLB5VH35JWZoCo_P6FsFq

12

https://books.google.iq/books?id=0XA7DwAAQBAJ&pg=PA224&lpg=PA224&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=W5EvtEHyBv&sig=Xj8oJw7ERgc4iDdHa1Ogkv8H5Sg&hl=en&sa=X&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChDoAQg7MAQ

13

https://books.google.iq/books?id=6cjgo1IixvEC&pg=PA885&lpg=PA885&dq=check+plant+extract+has+no+fungal+contamination&source=bl&ots=znBmPt4k3c&sig=0bXWTAB1y39SpxdUV6BpJ1aC-sg&hl=en&sa=X&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChDoAQg9MAU

14

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCEAwBg&url=http%3A%2F%2Fwww.imedpub.com%2Farticles%2Fplant-materials-as-probable-growth-promoters-for-certain-fungi.pdf&usg=AOvVaw2E785wteZ7jQ5EwovmnXCI

15

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCEgwBw&url=https%3A%2F%2Fwww.emlab.com%2Fmedia%2Fresources%2FAssessment-Fungal-Contamination-Buildings.pdf&usg=AOvVaw1p7BYqaEk3IwSmVNPppB--

16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCFEwCA&url=http%3A%2F%2Faem.asm.org%2Fcontent%2F74%2F23%2F7391.full&usg=AOvVaw3CijAYNhijmJwfyInA2bVd

17

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=20&cad=rja&uact=8&ved=0ahUKEwj784uL_JDYAhULCcAKHRQMACQ4ChAWCFkwCQ&url=http%3A%2F%2Fwww.journalrepository.org%2Fmedia%2Fjournals%2FARJA_47%2F2016%2FAug%2FFernandez122016ARJA28353.pdf&usg=AOvVaw1IsS23sguSfgN15Aw_26Cm

18

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=21&cad=rja&uact=8&ved=0ahUKEwjU_vL6_ZDYAhVoIsAKHSTYBkg4FBAWCCYwAA&url=https%3A%2F%2Fwww.hindawi.com%2Fjournals%2Fijmicro%2F2017%2F5273893%2F&usg=AOvVaw3K_VZnLj2R-M-rofWTCNIq

19

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=22&cad=rja&uact=8&ved=0ahUKEwjU_vL6_ZDYAhVoIsAKHSTYBkg4FBAWCC4wAQ&url=http%3A%2F%2Fjfoodprotection.org%2Fdoi%2Fpdf%2F10.4315%2F0362-028X-68.4.815&usg=AOvVaw1nwRdOtRSs7TGU6ltTQrOn

20

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=23&cad=rja&uact=8&ved=0ahUKEwjU_vL6_ZDYAhVoIsAKHSTYBkg4FBAWCDMwAg&url=http%3A%2F%2Fpubmedcentralcanada.ca%2Fpmcc%2Farticles%2FPMC1388767%2Fpdf%2Fhw0415.pdf&usg=AOvVaw1YWz_-nzY9_vpYxpkn_061