What is the best and cheapest methods to measure ABA or ethylene in roots? Or which parameter is best to measure for both salinity anoxia stress?

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How to remove fatty material from plant extract?

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Ram Nath Mishra

The following two reference may serve your purpose.

1. Current methods for detecting ethylene in plants

aob.oxfordjournals.org/content/early/2012/12/12/aob.mcs259.full

by SM Cristescu - ‎2012

Therefore the search for suitable ethylene measuring methods for various organ (e.g. root, leaf and flower) and developmental stage of the plant. High selectivity, good at separating complex mixtures and .... Obviously, the main considerations for this choice were their low cost, small size

2. Effects of oxygen tension, temperature, salinity, and humidity ...

www.int-res.com/articles/meps/32/m032p027.pdf

E. pirloti was more tolerant of anoxia, high temperature and low salinity similar to those recorded on the shore, but both species were able to survive ... that, of 6 parameters studied, the PO2, temperature, ... greatest seasonal and daily fluctuations, especially ... measured in air and water only at 25 and 30°C, and.

Juan Carlos Melgar

I have measured ABA with ELISA test. There are kits that make it quite simple to measure (I have bought them from Agdia Phytodetek) although they are not so cheap... about $500 a kit for about 80 samples (40, if you use two replicates/sample). You can measure it by using GC-MS... it's much cheaper, the only thing is that you would need to create a good calibration curve.

Indira Paudel

Thank you so much! Dear Juan Carlos and Ram nath Mishra.

I have found ELISA method also, looks expensive. As here in israel around $1000 for the price of KIT. So I am Searching anybody who have measure using GC-MS. Do You have any idea?

I dont know if the following papers would be of use to you.

Comprehensive chemical derivatization for gas chromatography–mass spectrometry-based multi-targeted profiling of the major phytohormones

C Birkemeyer, A Kolasa, J Kopka - Journal of Chromatography A, 2003 - Elsevier

selection of the N-methyl-N-(tert.-butyldimethylsilyl)

trifluoroacetamide (MTBSTFA) reagent as the most comprehensive derivatization protocol

A method for profiling classes of plant hormones and their metabolites using liquid chromatography‐electrospray ionization tandem mass spectrometry:

SDS Chiwocha, SR Abrams, SJ Ambrose… - The Plant …, 2003 - Wiley Online Library

Summary A highly selective and sensitive method for the simultaneous analysis of several plant hormones and their metabolites is described. The method combines high-performance liquid chromatography (HPLC) with positive and negative electrospray ionization-tandem .