What is the best alternative for Lyophilization of the sample before starting extraction in natural products?
Dear Safia,
I guess that depends on what extraction process you will use, and what effect water will have in the following processes - typically separation and analysis of the extracts. Freeze drying is simple, inexpensive and relatively easy, well accepted, but time consuming. It can help remove water whtat will impede your progress in later stages of your work. Alternatively, I have seen various extraction processes where water at >80°C is on extraction stage, often followed by an extraction with dichloromethane of the same substrate. Wet DCM is a challenge to dry / concenrate but not the worst.
Then answer to "best" really depends on what process you are using, and the limits / challenges of that process.
The following links to pages / papers may help:
https://www.spscientific.com/ContentBlock.aspx?id=3317
https://www.spscientific.com/Workarea/DownloadAsset.aspx?id=3958
Kind regards,
Rob
Hello, what is your substrate for lyophilization, and what future analytes will be?
Karen A. Darbinyan Karen A. Darbinyan Hello, the 'substrate' for lyophilization are fragments of a marine sponge, which further have to be extracted using solvents of varying polarities.
I think a simple adequate drying will be a good alternative of pre-treatment of the sample before extraction.
Hello you you can just dry the samples under vacuum if you need fast drying under low temperature or you can freeze the samples till analysis.
Abderrahim Benkhaled I agree that simple drying is the simplest option, but sometimes it takes excruciatingly long time.
Yes just freeze. For future and for real comparison of results you can use part dried, part frozen. And after that you will also have results for storage.
Karen A. Darbinyan Hello,
Well, I can't do that because I basically need to do extraction using different solvents, and the presence of water causes a lot of bumping when I dry the extract on rotary evaporator.
Safia Khan
You can use a drying in a microwave that is cheaper and faster
This method helps to obtain a powdered sample, a under normal conditions this is not possible.
Hi Safia
This reference may be useful
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwjbm42lh-_fAhUF2KQKHZW3Dg0QFjACegQICBAC&url=http%3A%2F%2Fwww.biomedsearch.com%2Fattachments%2F00%2F18%2F92%2F52%2F18925285%2FJAMMC-12-267.pdf&usg=AOvVaw1EKMyed5eEM9d8-viYeC3b
Ref.2
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=2ahUKEwjbm42lh-_fAhUF2KQKHZW3Dg0QFjADegQIBxAC&url=http%3A%2F%2Fwww.mdpi.com%2F1420-3049%2F17%2F9%2F10344%2Fpdf&usg=AOvVaw1wcMZB7OL-SZO1dJzGe6_j
Ref.3
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=2ahUKEwjbm42lh-_fAhUF2KQKHZW3Dg0QFjAEegQIBBAC&url=https%3A%2F%2Fpdfs.semanticscholar.org%2Fd9f4%2Fa4b942c8ecaa128daeb1eeb4fde95a80d1e9.pdf&usg=AOvVaw0JX1LMRYk2MQObhuTD9Zpm
Each drying method has advantages and disadvantages. If not the cost, change the texture. If not the loss of valuable ingredients, it is a shrinkage. Regards,
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction