15 January 2018 1 3K Report

Pond scale visual estimate (not small scale)-5 to 10 meters square.

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Publication duplicating concers?

Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...

25 June 2024 5,799 0 View

What learning analytics model is the best when it comes to improving student performance under any condition?

There is research into the models individually, but not one seeking to find an overall best one suited for any condition.

23 June 2024 5,641 3 View

How can I purify filamentous fungi from leaf tissue?

I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...

12 June 2024 4,027 1 View

Help me with plasmid concentration calculations?

Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...

10 June 2024 9,942 5 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

How to set up a PCR protocol?

I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...

28 February 2024 213 2 View

Genomic DNA qPCR protocol and analysis?

Hello All, I have several questions about qPCR. I will explain what I have done to give you a clear picture. I have 2 kidney samples. 1 wildtype and 1 KO for a specific gene. We have extracted...

25 February 2024 6,331 2 View

Relative quantification of genomic DNA qPCR?

I have extracted genomic DNA from 2 tissues. 1 wild type and 1 KO. I havd designed primers that should amplify a region in between the two loxp sites. I want to validate the effectiveness of the...

22 February 2024 9,660 0 View

How to represent raw qPCR data into a chart?

I have raw qPCR data. 2 samples "1 control and 1 with gene knock out". I made a serial dilution of each sample and performed a qPCR using 2 reference genes and 3 genes of interest. Now I have the...

21 February 2024 2,745 5 View

Separating Fe(II) complex from Fe(III) complex containing the same ligand?

Hi! Does anyone have ideas to separate Fe(II) complexes from Fe(III) complexes? Context about the complexes: 1) Coordination of the ligand causes some type of Fe(III) to Fe(II) reduction...

07 February 2024 8,464 2 View

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Does phosphorus limit phytoplankton growth and why does phosphorus limit primary production in freshwater lakes?

Does phosphorus limit phytoplankton growth and why does phosphorus limit primary production in freshwater lakes?

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Why have phytoplankton evolved inducible defenses?

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Why does iodine mix salt is harmful for the fresh water fish boy in aqaculture?

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Has there been a 40% decline in phytoplankton?

Nature 2010

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What is this strange cylindrical concretion in lacustrine sediment core ?

Dear all, we found these empty cylindrical cocncretions in severals ponds and lakes from different mountain range in Uzbekistan. Any ideas of what it could be ? The pictures have been took under...

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Good day sir/ma please can some one provide some hint for me about "is strip of view of microscope slide thesame with field of view"?

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How to separate solid particles from microbes(bacteria, phytoplankton, zooplankton) in aquatic chemistry analysis and species recognition levels?

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