What is the appropriate wavelength and dilution ratio for determining blood serum calcium and magnesium by Atomic absorption spectrometry?

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Jukka Salminen

If you use a hollow cathode lamp you already have the wavelength set. Other details are described in the litterature.

clinchem.aaccjnls.org/content/clinchem/13/2/101.full.pdf

https://www.theseus.fi/bitstream/handle/10024/47483/heini_makinen.pdf?sequence=1

https://pdfs.semanticscholar.org/72a3/f71892a409ed269983166abaa02527b1a9d0.pdf

Jos Wielders

You can also read these application notes below.

https://www.agilent.com/cs/library/applications/aa117.pdf

https://airccse.com/ijbes/papers/1214ijbes05.pdf

Some practical points: Always filter the diluent before use. Clean nebulizer after use, also the narrow inlet with a thread. A corroded nebulizer chamber has to be replaced. Check the needed air pressure from your AAS user manual and make sure that your air supply has enough pressure and does not fluctuate. Aspirate some sample before calibration so that the chamber gets wetted. At the same time check burner height and air flow (affects repeatability).

Amir Khaki

Thank you so much for these merit practical points.