High fit and low fit individuals were asked to complete a bout of exercise and blood draws were taken PRE, POST, 1HR, and 2HR. Each blood draw was placed in culture and stimulated with a high lipid concentration (or not, unstimulated) as an in vitro model of postprandial lipemia. We would like to determine if the response of the macrophages to the lipid stimulus is A) different following exercise B) if the impact of exercise is moderated by fitness level.
These analyses must take into account within-subject factors (time and condition) as well as the between-subject factor (fitness).